Browsing by Author "Taxa, Luis"

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Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR
(Publmed, 2020) Castaneda, Carlos A; Castillo, Miluska; Sanchez, Joselyn; Casavilca, Sandro; Sanchez, Juvenal; Bernabe, Luis A; Suarez, Nancy; Chavez, Ivan; Ruiz, Eloy; Tello, Katherine; Villa, Maria R; Zevallos, Rocio; Montenegro, Paola; Dias-Neto, Emmanuel; Landa-Baella, Maria; Taxa, Luis
Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results:H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.
Level of tumor-infiltrating lymphocytes and density of infiltrating immune cells in different malignancies
(Publmed, 2019) Castañeda, Carlos A.; Castillo, Miluska; Aliaga, Karina; Bernabé, Luis A.; Casavilca, Sandro; Sánchez, Joselyn; Torres-Cabala, Carlos A.; Gómez , Henry L.; Mas, Luis; Dunstan, Jorge; Cotrina, José M; Abugattas, Julio; Chávez, Iván; Ruiz, Eloy; Montenegro, Paola; Rojas, Víctor; Orrego, Enrique; Gálvez-Nino, Marco; Félix , Brayam; Landa-Baella, María P.; Vidaurre, Tatiana; Villa, María R; Zevallos, Rocío; Taxa, Luis; Guerra, Henry; Jorge Luis
Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International Immuno-Oncology Biomarker Working Group methodology, and both density of tumor-infiltrating immune cell and clinicopathological features in different malignancies. Methods: 209 pathological samples from gastric cancer, cervical cancer (CC), non-small-lung cancer, cutaneous melanoma (CM) and glioblastoma were tested for TIL in hematoxylin eosin, and density of CD3+, CD4+, CD8+, CD20+, CD68+ and CD163+ cells by digital analysis. Results: TIL levels were higher in invasive margin compartments (IMC). TIL in IMC, intratumoral and stromal compartments predicted survival. CC and gastric cancer had higher TIL in intratumoral; CC and CM had higher TIL in stromal compartment and IMC. CM had the highest density of lymphocyte and macrophage populations. CD20 density was associated with survival in the whole series. Conclusion: Standardized evaluation of TIL levels may provide valuable prognostic information in a spectrum of different malignancies.
Metallomic profile in non-cirrhotic hepatocellular carcinoma supports a phenomenon of metal metabolism adaptation in tumor cells
(Nature, 2021) Cano, Luis; Bertani, Stéphane; Island, Marie-Laure; Cerapio, Juan Pablo; Ruiz, Eloy; Pineau, Pascal; Monbet, Valérie; Boudjema, Karim; Taxa, Luis; Casavilca-Zambrano, Sandro; Ropert, Martine; Turlin, Bruno; Loréal, Olivier
We have previously described a form of hepatocellular carcinoma (HCC) in non-cirrhotic liver (HCC-NC) developed by Peruvian patients. We analyzed the metallomic profile in hepatic tissues from two independent cohorts exhibiting HCC-NC. Clinical, histopathological data, and HCC and non-tumoral liver (NTL) samples of 38 Peruvian and 38 French HCC-NC patients, were studied. Twelve metals were quantified using ICP/MS: Mn, Fe, Cu, Co, Zn, As, Se, Rb, Mo, Cd, Pb, and Sn. Associations between metals and survival were assessed. Our data showed significant differences between cohorts. Mean ages were 40.6 ± 20, 67.5 ± 9 years old for Peruvians and French, respectively. Fifty percent of the Peruvian patients were positive for the HBsAg, versus 3% in French patients. Mn, Cu, Zn, As, Se, Rb, Mo, Cd, Sn metal concentrations were higher in NTL of Peruvians. Importantly, metal concentrations were lower in HCC areas compared to NTL tissues in both cohorts, except for Cu for which mean concentration was higher in HCC (p < 0.05). Se concentration in HCC was associated with extended survival only in Peruvians. Our data, obtained in Peruvian and French HCC-NC cohorts, highlights similarity in the metallomic profile of HCC compared to NTL during the hepatic tumorigenesis in these specific groups of patients.