Browsing by Author "Ruiz, Eloy"

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    Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR
    (Publmed, 2020) Castaneda, Carlos A; Castillo, Miluska; Sanchez, Joselyn; Casavilca, Sandro; Sanchez, Juvenal; Bernabe, Luis A; Suarez, Nancy; Chavez, Ivan; Ruiz, Eloy; Tello, Katherine; Villa, Maria R; Zevallos, Rocio; Montenegro, Paola; Dias-Neto, Emmanuel; Landa-Baella, Maria; Taxa, Luis
    Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results:H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.
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    Level of tumor-infiltrating lymphocytes and density of infiltrating immune cells in different malignancies
    (Publmed, 2019) Castañeda, Carlos A.; Castillo, Miluska; Aliaga, Karina; Bernabé, Luis A.; Casavilca, Sandro; Sánchez, Joselyn; Torres-Cabala, Carlos A.; Gómez , Henry L.; Mas, Luis; Dunstan, Jorge; Cotrina, José M; Abugattas, Julio; Chávez, Iván; Ruiz, Eloy; Montenegro, Paola; Rojas, Víctor; Orrego, Enrique; Gálvez-Nino, Marco; Félix , Brayam; Landa-Baella, María P.; Vidaurre, Tatiana; Villa, María R; Zevallos, Rocío; Taxa, Luis; Guerra, Henry; Jorge Luis
    Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International Immuno-Oncology Biomarker Working Group methodology, and both density of tumor-infiltrating immune cell and clinicopathological features in different malignancies. Methods: 209 pathological samples from gastric cancer, cervical cancer (CC), non-small-lung cancer, cutaneous melanoma (CM) and glioblastoma were tested for TIL in hematoxylin eosin, and density of CD3+, CD4+, CD8+, CD20+, CD68+ and CD163+ cells by digital analysis. Results: TIL levels were higher in invasive margin compartments (IMC). TIL in IMC, intratumoral and stromal compartments predicted survival. CC and gastric cancer had higher TIL in intratumoral; CC and CM had higher TIL in stromal compartment and IMC. CM had the highest density of lymphocyte and macrophage populations. CD20 density was associated with survival in the whole series. Conclusion: Standardized evaluation of TIL levels may provide valuable prognostic information in a spectrum of different malignancies.
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    Metallomic profile in non-cirrhotic hepatocellular carcinoma supports a phenomenon of metal metabolism adaptation in tumor cells
    (Nature, 2021) Cano, Luis; Bertani, Stéphane; Island, Marie-Laure; Cerapio, Juan Pablo; Ruiz, Eloy; Pineau, Pascal; Monbet, Valérie; Boudjema, Karim; Taxa, Luis; Casavilca-Zambrano, Sandro; Ropert, Martine; Turlin, Bruno; Loréal, Olivier
    We have previously described a form of hepatocellular carcinoma (HCC) in non-cirrhotic liver (HCC-NC) developed by Peruvian patients. We analyzed the metallomic profile in hepatic tissues from two independent cohorts exhibiting HCC-NC. Clinical, histopathological data, and HCC and non-tumoral liver (NTL) samples of 38 Peruvian and 38 French HCC-NC patients, were studied. Twelve metals were quantified using ICP/MS: Mn, Fe, Cu, Co, Zn, As, Se, Rb, Mo, Cd, Pb, and Sn. Associations between metals and survival were assessed. Our data showed significant differences between cohorts. Mean ages were 40.6 ± 20, 67.5 ± 9 years old for Peruvians and French, respectively. Fifty percent of the Peruvian patients were positive for the HBsAg, versus 3% in French patients. Mn, Cu, Zn, As, Se, Rb, Mo, Cd, Sn metal concentrations were higher in NTL of Peruvians. Importantly, metal concentrations were lower in HCC areas compared to NTL tissues in both cohorts, except for Cu for which mean concentration was higher in HCC (p < 0.05). Se concentration in HCC was associated with extended survival only in Peruvians. Our data, obtained in Peruvian and French HCC-NC cohorts, highlights similarity in the metallomic profile of HCC compared to NTL during the hepatic tumorigenesis in these specific groups of patients.
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    Natural Occurrence of Mycotoxin-Producing Fusaria in Market-Bought Peruvian Cereals: A Food Safety Threat for Andean Populations
    (MDPI, 2021) Ducos, Christine; Pinson-Gadais, Laetitia; Chereau, Sylvain; Richard-Forget, Florence; Vásquez-Ocmín, Pedro; Cerapio, Juan Pablo; Casavilca-Zambrano, Sandro; Ruiz, Eloy; Pineau, Pascal; Bertani, Stéphane; Ponts, Nadia
    Consumption of cereals contaminated by mycotoxins poses health risks. For instance, Fumonisins B, mainly produced by Fusarium verticillioides and Fusarium proliferatum, and the type B trichothecene deoxynivalenol, typically produced by Fusarium graminearum, are highly prevalent on cereal grains that are staples of many cultural diets and known to represent a toxic risk hazard. In Peru, corn and other cereals are frequently consumed on a daily basis under various forms, the majority of food grains being sold through traditional markets for direct consumption. Here, we surveyed mycotoxin contents of market-bought grain samples in order to assess the threat these mycotoxins might represent to Peruvian population, with a focus on corn. We found that nearly one sample of Peruvian corn out of six was contaminated with very high levels of Fumonisins, levels mostly ascribed to the presence of F. verticillioides. Extensive profiling of Peruvian corn kernels for fungal contaminants could provide elements to refine the potential risk associated with Fusarium toxins and help define adapted food safety standards.
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    Prevalence of H. pylori Infection in Relatives of Peruvian Patients with Gastric Cancer
    (Asian Pacific Journal of Cancer Care, 2021) Castillo, Miluska; Bernabe, Luis A.; Castaneda, Carlos A.; Suarez, Nancy; Barreda, Fernando; Valdivia, Daniel; Ruiz, Eloy; Nieves, Jais; Dias-Neto, Emmanuel; Landa-Baella, Maria; Montano-Flores, Jennifer; Chavez, Ivan
    bjective: The aim of this study was to determine the relationship between Helicobacter pylori (H. pylori) infection in relatives and patients with gastric cancer (GC). Methods: H. pylori infection was evaluated by the breath urease test in 171 relatives and by qPCR technique in gastric tissue of 61 patients (n=45 for GC and n=16 for non-GC). Results: There were included 137 relatives of GC patients and 34 of non-GC. The median age of the relatives of patients with a gastric tissue sample was 39 years (10-86). Infection was found in 60.2% (n=103) relatives. There were no higher H. pylori infection rates in relatives of patients with gastric cancer (62% vs 62.9%, p=0.33), H. pylori infection (60% vs 60%, p=0.96), or metaplasia (58.8% vs 61.8%, p=0.71). Conclusion: The prevalence of infection in relatives of GC patients is high in our population but not associated with H. pylori presence in the paired case