Browsing by Author "Casavilca, Sandro"

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Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR
(Publmed, 2020) Castaneda, Carlos A; Castillo, Miluska; Sanchez, Joselyn; Casavilca, Sandro; Sanchez, Juvenal; Bernabe, Luis A; Suarez, Nancy; Chavez, Ivan; Ruiz, Eloy; Tello, Katherine; Villa, Maria R; Zevallos, Rocio; Montenegro, Paola; Dias-Neto, Emmanuel; Landa-Baella, Maria; Taxa, Luis
Aim:Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results:H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.
Distribution of tumor-infiltrating immune cells in glioblastoma
(Future Medicine Ltd., 2018) Orrego Puelles, Enrique,; Castaneda, Carlos A; Castillo, Miluska; Bernabe, LA; Casavilca, Sandro; Chakravarti, A; Meng, W; Garcia-Corrochano, Pamela; Villa-Robles, MR; Zevallos, R; Mejia, O; Deza, Pedro; Belmar-Lopez; Ojeda, CL
Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.
Level of tumor-infiltrating lymphocytes and density of infiltrating immune cells in different malignancies
(Publmed, 2019) Castañeda, Carlos A.; Castillo, Miluska; Aliaga, Karina; Bernabé, Luis A.; Casavilca, Sandro; Sánchez, Joselyn; Torres-Cabala, Carlos A.; Gómez , Henry L.; Mas, Luis; Dunstan, Jorge; Cotrina, José M; Abugattas, Julio; Chávez, Iván; Ruiz, Eloy; Montenegro, Paola; Rojas, Víctor; Orrego, Enrique; Gálvez-Nino, Marco; Félix , Brayam; Landa-Baella, María P.; Vidaurre, Tatiana; Villa, María R; Zevallos, Rocío; Taxa, Luis; Guerra, Henry; Jorge Luis
Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International Immuno-Oncology Biomarker Working Group methodology, and both density of tumor-infiltrating immune cell and clinicopathological features in different malignancies. Methods: 209 pathological samples from gastric cancer, cervical cancer (CC), non-small-lung cancer, cutaneous melanoma (CM) and glioblastoma were tested for TIL in hematoxylin eosin, and density of CD3+, CD4+, CD8+, CD20+, CD68+ and CD163+ cells by digital analysis. Results: TIL levels were higher in invasive margin compartments (IMC). TIL in IMC, intratumoral and stromal compartments predicted survival. CC and gastric cancer had higher TIL in intratumoral; CC and CM had higher TIL in stromal compartment and IMC. CM had the highest density of lymphocyte and macrophage populations. CD20 density was associated with survival in the whole series. Conclusion: Standardized evaluation of TIL levels may provide valuable prognostic information in a spectrum of different malignancies.